Immunogram

Investigate beyond PD-L1

The arrival of immune checkpoint (ICP) inhibitors in biopharma’s development pipelines has brought many changes to the oncology clinical trial landscape. Should it be for monotherapy or for combination study, all patients entering an ICP inhibitor clinical trial should be characterized, not only for PD-L1 expression but for as many ICP inhibitor related biomarkers as possible.
immunogram PD-L1

Five parameters

At OncoDNA we have developed a 5 parameters immunogram, combining the testing of:

  • The level of expression of PD-L1
  • The level of expression of CD8 in Lymphocytes
  • The tumor mutational burden (TMB)
  • The microsatellite instability (MSI)
  • The presence of mutations associated with resistance or sensitivity to ICP inhibitors.

 

immunogram PD-L1

5 parameters immunogram

Expression of PD-L1

PD-l1

This is the first FDA approved biomarker. If several antibodies exist to detect PD-L1 expression, only PD-L1 IHC 22C3 pharmDx (Dako) is approved as a companion diagnostic for pembrolizumab. Dako’s PD-L1 IHC 28-8 pharmDx is approved as a complementary diagnostic for nivolumab. These two antibodies and others are available at OncoDNA.

CD8 T-cell infiltrate as detected by IHC

CD8

The presence of CD8+ lymphocytes has been associated with better clinical outcomes for patients treated with ICP inhibitors.

The tumor mutational burden (TMB)

TMB

TMB is a measurement of the mutations carried by tumor cells (number of mutation by units of coding area) and it correlates with higher level of neoantigens. High TMB has been associated to better response and increased survival rate for patients treated with ICP inhibitors.

The microsatellite instability (MSI)

MSI

MSI, a type of genomic instability, is characterized by gains or losses of nucleotides from microsatellite tracts. It results from impaired DNA mismatch repair (MMR) and MSI-H(igh) status has been associated with high response rates and survival benefit for patients treated with checkpoint inhibitors.

Mutations associated with sensitivity or resistance to ICP inhibitors

MUT

Mutations associated with sensitivity (e.g. POLE inactivating mutations) or resistance (e.g. STK11 inactivating mutations) to ICP inhibitors. We can test this continuously growing number of variants associated with an impact on immunotherapy.

The Most Comprehensive Genomic and protein based solution

This immunogram is the most comprehensive genomic and protein based solution on the market. But it can of course be tailored according to our biopharma customers’ specific needs, including any other molecular characterization parameters such as IHC tested CD3 or PD-L2 expression, RNA-Seq or Whole Exome Sequencing.

In the case of combination studies, in addition to the Immunogram, we can also provide comprehensive molecular characterization of patients using DNA/NGS (for targeted therapies) or IHCs (for chemotherapies) characterization.

Your benefits

  • The most comprehensive approach to characterize ICP inhibitor treated patients.
  • Customizable according to customer’s specific needs.
  • Multifactor and integrative approach all with a single partner.
  • One stop shop solution for combination studies.
A question about our Immunogram?